Fetching sequence for NM_001256799...
Fetching exon annotation for NM_001256799...
Found 7 exon junctions.
Searching for highly specific primer/probe sets...
Found 5 valid assay designs. Showing top 3:
[
  {
    "Amplicon_Size": 171,
    "gDNA_Safe": "Yes",
    "F_Primer": {
      "seq": "GGAAATGAATGGGCAGCCG",
      "tm": 60.0,
      "len": 19
    },
    "R_Primer": {
      "seq": "CCAATACGACCAAATCAGAGAA",
      "tm": 62.0,
      "len": 22
    },
    "Probe": {
      "seq": "CCCTGCGCTCCTGCCTCG",
      "tm": 64.0,
      "len": 18
    }
  },
  {
    "Amplicon_Size": 170,
    "gDNA_Safe": "Yes",
    "F_Primer": {
      "seq": "GAAATGAATGGGCAGCCGT",
      "tm": 58.0,
      "len": 19
    },
    "R_Primer": {
      "seq": "CCAATACGACCAAATCAGAGA",
      "tm": 60.0,
      "len": 21
    },
    "Probe": {
      "seq": "ACCCTGCGCTCCTGCCTC",
      "tm": 62.0,
      "len": 18
    }
  },
  {
    "Amplicon_Size": 170,
    "gDNA_Safe": "Yes",
    "F_Primer": {
      "seq": "GAAATGAATGGGCAGCCGT",
      "tm": 58.0,
      "len": 19
    },
    "R_Primer": {
      "seq": "CCAATACGACCAAATCAGAGAA",
      "tm": 62.0,
      "len": 22
    },
    "Probe": {
      "seq": "ACCCTGCGCTCCTGCCTC",
      "tm": 62.0,
      "len": 18
    }
  }
]
